Studies on the Chromatography on Polyamide. I. : Thin-Layer Chromatography on Polyamide

Polyamide thin-layer chromatography of phosphorylated tyrosine, threonine, and serine.

One-dimensional thin-layer chromatography on polyamide plates offers an easy and rapid identification of O-phosphotyrosine. The thin-layer plate is developed for 30 min in 5% propionic acid containing 0.013%-0.025% sodium dodecyl sulfate. O-Phosphotyrosine, with Rf = 0.54, can be well separated from O-phosphothreonine and O-phosphoserine, which comigrate at Rf = 0.72.

Unidimensional Thin Layer Chromatography of Salicylic Acid Derivatives on Boric Acid-Impregnated Plates in Drugs Studies

A unidimensional Thin Layer Chromatography system for the separation of salicylic acid and its derivatives (sulfosalicylic acid and Aspirin) has been developed. The separation was successfully using a solvent system of chloroform-methanol-water-ammonium hydroxide 120:75:6:2 (V/V) as mobile phase on TLC plates impregnated with boric acid. The method is based on different behavior of investigated...

The Lipophilicity Examination of Some ACE inhibitors and Hydrochlorothiazide on Cellulose in RP Thin-Layer Chromatography

In this assay, the evaluation of lipophilicity of four ACE-inhibitors and hydrochlorothiazide (HCTZ) with RP-TLC on cellulose layers was described using three binary solvent systems. The selected ACE inhibitors had sufficiently different structures which can indicate the method suitability for their lipophilicity evaluation as the model substances in comparison with HCTZ. In addition, the linea...

The Lipophilicity Examination of Some ACE inhibitors and Hydrochlorothiazide on Cellulose in RP Thin-Layer Chromatography

In this assay, the evaluation of lipophilicity of four ACE-inhibitors and hydrochlorothiazide (HCTZ) with RP-TLC on cellulose layers was described using three binary solvent systems. The selected ACE inhibitors had sufficiently different structures which can indicate the method suitability for their lipophilicity evaluation as the model substances in comparison with HCTZ. In addition, the linea...

unidimensional thin layer chromatography of salicylic acid derivatives on boric acid-impregnated plates in drugs studies